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qbase relative quantification analysis software  (Biogazelle)

 
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    Structured Review

    Biogazelle qbase relative quantification analysis software
    Image <t>analysis</t> of Masson's trichrome‐stained lung sections for the <t>quantification</t> of airway smooth muscle (ASM; C) and collagen content (D) <t>relative</t> to basement membrane (BM) length. Anti‐tryptase mast cell+ staining (white arrows; degranulated mast cells indicated with open arrows) in lung tissue of control (E) and allergic pregnant sheep (F), with cell counts presented in airway parenchyma (G) and within the airway wall (H). Differences between control and allergic animals are shown by **P < 0.01. Quantitative data are presented as data from individual animals for control (open circles) and allergic (filled circles) sheep, with means ± SEM (n = 5/group).
    Qbase Relative Quantification Analysis Software, supplied by Biogazelle, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/qbase+relative+quantification+analysis+software/qbase+relative+quantification+software/pmc04771800-199-51-48
    Average 90 stars, based on 1 article reviews
    qbase relative quantification analysis software - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Development of an experimental model of maternal allergic asthma during pregnancy"

    Article Title: Development of an experimental model of maternal allergic asthma during pregnancy

    Journal: The Journal of Physiology

    doi: 10.1113/JP270752

    Image analysis of Masson's trichrome‐stained lung sections for the quantification of airway smooth muscle (ASM; C) and collagen content (D) relative to basement membrane (BM) length. Anti‐tryptase mast cell+ staining (white arrows; degranulated mast cells indicated with open arrows) in lung tissue of control (E) and allergic pregnant sheep (F), with cell counts presented in airway parenchyma (G) and within the airway wall (H). Differences between control and allergic animals are shown by **P < 0.01. Quantitative data are presented as data from individual animals for control (open circles) and allergic (filled circles) sheep, with means ± SEM (n = 5/group).
    Figure Legend Snippet: Image analysis of Masson's trichrome‐stained lung sections for the quantification of airway smooth muscle (ASM; C) and collagen content (D) relative to basement membrane (BM) length. Anti‐tryptase mast cell+ staining (white arrows; degranulated mast cells indicated with open arrows) in lung tissue of control (E) and allergic pregnant sheep (F), with cell counts presented in airway parenchyma (G) and within the airway wall (H). Differences between control and allergic animals are shown by **P < 0.01. Quantitative data are presented as data from individual animals for control (open circles) and allergic (filled circles) sheep, with means ± SEM (n = 5/group).

    Techniques Used: Staining, Membrane, Control

    Related Articles

    SYBR Green Assay:

    Article Title: Conditional Involvement of CONSTITUTIVE PHOTOMORPHOGENIC1 in the Degradation of Phytochrome A 1 [W] [OA]
    Article Snippet: R Green PCR master mix from Applied Biosystems using the ABI Prism 7900 HT Sequence Detection System according to the manufacturer’s instructions. For the relative quantification of the genes, we used qBase software for the management and automated analysis of real-time PCR ( http://medgen.ugent.be/qbase ). Each reaction was performed in triplicate using a primer concentration of 300 n m . EF1α (At

    Real-time Polymerase Chain Reaction:

    Article Title: Conditional Involvement of CONSTITUTIVE PHOTOMORPHOGENIC1 in the Degradation of Phytochrome A 1 [W] [OA]
    Article Snippet: R Green PCR master mix from Applied Biosystems using the ABI Prism 7900 HT Sequence Detection System according to the manufacturer’s instructions. For the relative quantification of the genes, we used qBase software for the management and automated analysis of real-time PCR ( http://medgen.ugent.be/qbase ). Each reaction was performed in triplicate using a primer concentration of 300 n m . EF1α (At

    Polymerase Chain Reaction:

    Article Title: Conditional Involvement of CONSTITUTIVE PHOTOMORPHOGENIC1 in the Degradation of Phytochrome A 1 [W] [OA]
    Article Snippet: R Green PCR master mix from Applied Biosystems using the ABI Prism 7900 HT Sequence Detection System according to the manufacturer’s instructions. For the relative quantification of the genes, we used qBase software for the management and automated analysis of real-time PCR ( http://medgen.ugent.be/qbase ). Each reaction was performed in triplicate using a primer concentration of 300 n m . EF1α (At

    Quantitative Proteomics:

    Article Title: Conditional Involvement of CONSTITUTIVE PHOTOMORPHOGENIC1 in the Degradation of Phytochrome A 1 [W] [OA]
    Article Snippet: R Green PCR master mix from Applied Biosystems using the ABI Prism 7900 HT Sequence Detection System according to the manufacturer’s instructions. For the relative quantification of the genes, we used qBase software for the management and automated analysis of real-time PCR ( http://medgen.ugent.be/qbase ). Each reaction was performed in triplicate using a primer concentration of 300 n m . EF1α (At

    Software:

    Article Title: Conditional Involvement of CONSTITUTIVE PHOTOMORPHOGENIC1 in the Degradation of Phytochrome A 1 [W] [OA]
    Article Snippet: R Green PCR master mix from Applied Biosystems using the ABI Prism 7900 HT Sequence Detection System according to the manufacturer’s instructions. For the relative quantification of the genes, we used qBase software for the management and automated analysis of real-time PCR ( http://medgen.ugent.be/qbase ). Each reaction was performed in triplicate using a primer concentration of 300 n m . EF1α (At

    Sequencing:

    Article Title: Conditional Involvement of CONSTITUTIVE PHOTOMORPHOGENIC1 in the Degradation of Phytochrome A 1 [W] [OA]
    Article Snippet: R Green PCR master mix from Applied Biosystems using the ABI Prism 7900 HT Sequence Detection System according to the manufacturer’s instructions. For the relative quantification of the genes, we used qBase software for the management and automated analysis of real-time PCR ( http://medgen.ugent.be/qbase ). Each reaction was performed in triplicate using a primer concentration of 300 n m . EF1α (At

    Concentration Assay:

    Article Title: Conditional Involvement of CONSTITUTIVE PHOTOMORPHOGENIC1 in the Degradation of Phytochrome A 1 [W] [OA]
    Article Snippet: R Green PCR master mix from Applied Biosystems using the ABI Prism 7900 HT Sequence Detection System according to the manufacturer’s instructions. For the relative quantification of the genes, we used qBase software for the management and automated analysis of real-time PCR ( http://medgen.ugent.be/qbase ). Each reaction was performed in triplicate using a primer concentration of 300 n m . EF1α (At



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    Biogazelle qbase relative quantification analysis software
    Image <t>analysis</t> of Masson's trichrome‐stained lung sections for the <t>quantification</t> of airway smooth muscle (ASM; C) and collagen content (D) <t>relative</t> to basement membrane (BM) length. Anti‐tryptase mast cell+ staining (white arrows; degranulated mast cells indicated with open arrows) in lung tissue of control (E) and allergic pregnant sheep (F), with cell counts presented in airway parenchyma (G) and within the airway wall (H). Differences between control and allergic animals are shown by **P < 0.01. Quantitative data are presented as data from individual animals for control (open circles) and allergic (filled circles) sheep, with means ± SEM (n = 5/group).
    Qbase Relative Quantification Analysis Software, supplied by Biogazelle, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/qbase+relative+quantification+analysis+software/qbase+relative+quantification+software/pmc04771800-199-51-48
    Average 90 stars, based on 1 article reviews
    qbase relative quantification analysis software - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

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    Image analysis of Masson's trichrome‐stained lung sections for the quantification of airway smooth muscle (ASM; C) and collagen content (D) relative to basement membrane (BM) length. Anti‐tryptase mast cell+ staining (white arrows; degranulated mast cells indicated with open arrows) in lung tissue of control (E) and allergic pregnant sheep (F), with cell counts presented in airway parenchyma (G) and within the airway wall (H). Differences between control and allergic animals are shown by **P < 0.01. Quantitative data are presented as data from individual animals for control (open circles) and allergic (filled circles) sheep, with means ± SEM (n = 5/group).

    Journal: The Journal of Physiology

    Article Title: Development of an experimental model of maternal allergic asthma during pregnancy

    doi: 10.1113/JP270752

    Figure Lengend Snippet: Image analysis of Masson's trichrome‐stained lung sections for the quantification of airway smooth muscle (ASM; C) and collagen content (D) relative to basement membrane (BM) length. Anti‐tryptase mast cell+ staining (white arrows; degranulated mast cells indicated with open arrows) in lung tissue of control (E) and allergic pregnant sheep (F), with cell counts presented in airway parenchyma (G) and within the airway wall (H). Differences between control and allergic animals are shown by **P < 0.01. Quantitative data are presented as data from individual animals for control (open circles) and allergic (filled circles) sheep, with means ± SEM (n = 5/group).

    Article Snippet: The reference genes (β actin, hypoxanthine phosphoribosyltransferase 1 and tyrosine 3‐monooxygenase (YWAHZ)) were chosen (Duffield et al . 2009 ; Passmore et al . 2009 ; Wang et al . 2013 ; Hellemans & Vandesompele, 2014 ) based on expression analysis using the geNorm component of the qBase (Biogazelle, Zwinjnaarde, Belgium) relative quantification analysis software (Hellemans et al . 2007 ) because their expression was stable across samples (maximum value, factor = 0.3–0.4; Vandesompele et al . 2002 ; Soo et al . 2012 ; Hellemans & Vandesompele, 2014 ).

    Techniques: Staining, Membrane, Control